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相关概念视频

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...

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相关实验视频

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Published on: September 17, 2017

快速或缓慢的魔术角度定向样本旋转固态NMR光谱的双层样本.

Christina Sizun1, Burkhard Bechinger

  • 1Max-Planck-Institut für Biochemie, Am Klopferspitz 18A, 82152 Martinsried, Germany.

Journal of the American Chemical Society
|February 14, 2002
PubMed
概括
此摘要是机器生成的。

一个新的神奇的角度定向旋转光谱仪设置改善了脂质双层分析. 这种方法使用螺旋绕的聚合物薄膜来实现更窄的线宽和更高的旋转速度,增强核磁共振 (NMR) 能力.

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Published on: September 17, 2017

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科学领域:

  • 固态核磁共振 (NMR) 光谱学 固态核磁共振 (NMR) 光谱学
  • 生物物理化学 生物物理化学
  • 材料科学 材料科学 材料科学

背景情况:

  • 魔术角度定向旋转光谱 (MAOSS) 对于研究固态样本中的分子方向至关重要.
  • 使用堆叠玻璃板的传统MAOSS设置在旋转速度和线条宽度方面存在限制.
  • 对脂质双层的定位对于理解膜蛋白的结构和功能至关重要.

研究的目的:

  • 建议和评估用于魔术角度定向旋转光谱学 (MAOSS) 的替代设置.
  • 为了提高光谱分辨率和增加面向脂质双层样本可访问的旋转频率范围.
  • 为了使先进的高分辨率的多维NMR实验在面向的生物膜.

主要方法:

  • 脂质双层面向聚合物薄膜.
  • 带有定向双层的聚合物薄膜被包裹成螺旋状的配置.
  • 这个螺旋样本的几何形状被调整成适合标准的4或7毫米MAS旋转器.
  • 用各种旋转频率进行光谱分析.

主要成果:

  • 与传统的MAOSS设置相比,拟议的螺旋几何学导致线宽显著更窄.
  • 通过新的设置,实现了更高的上极限.
  • 从低旋转的光谱中成功地提取了定向信息.
  • 这些发现表明,它适用于高速旋转速度的高分辨率多维NMR脉冲序列.

结论:

  • 替代的MAOSS设置为面向的脂质双层样本提供了卓越的性能.
  • 这种新型几何学克服了传统方法的局限性,使得NMR研究得到了增强.
  • 改善的光谱质量和更高的旋转能力为使用固态NMR研究复杂的生物系统开辟了新的途径.