在和蛋白质中探测II型β转形成的结构决定因素
Alan C Gibbs1, Trent C Bjorndahl, Robert S Hodges
1Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8.
Journal of the American Chemical Society
|February 14, 2002
概括
对β-hairpin的结构分析揭示了稳定II型的关键因素.
科学领域:
- * * 结构生物学 结构生物学
- * 的化学结构
- * 计算式生物物理学
背景情况:
- * 贝塔毛刺是重要的蛋白质结构图案.
- *II型β转是β毛针内部的特定转结构.
- * 了解β转向稳定性对于蛋白质设计至关重要.
研究的目的:
- * 调查II'型β转稳定性的结构决定因素.
- *分析脊柱性,侧链相互作用和N-化对β转向稳定性的影响.
- *为预测β转向倾向和设计新提供洞察力.
主要方法:
- *使用循环二重化 (CD),核磁共振 (NMR) 和分子动力学模拟进行全面分析.
- *设计和合成10个定制的β-hairpin,使用系统的氨基酸替代.
- *评估β-sheet内容,分子动力学和3D结构分析.
主要成果:
- * 脊柱异构性占II型β转向稳定性的高达60%.
- *侧链N-化增强了β转向倾向,贡献了高达20%的稳定性.
- * 芳香/的侧链相互作用约为10%的稳定性.
结论:
- * 脊柱性和侧链修饰是II型β转向稳定的主要驱动因素.
- *这些发现有助于预测β转向倾向和的合理设计.
- * 这项研究有助于开发基于的药物和新型蛋白质设计.
相关概念视频
Protein Organization
Overview
Protein Folding
Overview
Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
Protein Folding
Overview
Protein Organization
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
The primary structure of a protein is its amino acid sequence.
Protein Folding
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...


