植物病原体Pseudomonas syringae的III型 (Hrp) 秘密体的功能性选
David S Guttman1, Boris A Vinatzer, Sara F Sarkar
1Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, ON M5S 3B2, Canada. guttman@botany.utoronto.ca
Science (New York, N.Y.)
|March 2, 2002
概括
研究人员确定了细菌类型III分泌的效应蛋白,这些蛋白对毒性至关重要. 一项新的遗传查和生物信息分析揭示了38种Pseudomonas syringae效应物,有助于理解植物病原体适应.
科学领域:
- 微生物学 微生物学
- 植物病理学 植物病理学
- 遗传学 是一个遗传学.
背景情况:
- 第三类分泌的效应蛋白对细菌病原体毒性至关重要.
- 鉴定这些效应因子是具有挑战性的,因为低同质性.
- Hrp (过敏反应和致病性) 装置有助于植物病原体中的效应因子分泌.
研究的目的:
- 为了识别来自Pseudomonas syringae的III型分泌效应蛋白.
- 开发一种用于预测新型效应者的方法.
- 研究效应因子在宿主适应中的作用.
主要方法:
- 在体内使用基因选来识别效应因子.
- 生物信息分析侧重于保存的氨基终端氨基酸组成.
- 对预测的效应因子进行了实验验证,对III型依赖性分泌物进行了验证.
主要成果:
- 鉴定出了由Pseudomonas注射器的HRP装置分泌的13种效应剂.
- 一个保存的氨基末端特征使38P.注射器效应者的生物信息预测成为可能.
- 确定了15个以前未知的效应因子,其中2个被证实是III型依赖性.
结论:
- 这项研究成功地确定并预测了P. syringae中的许多III型因子.
- 保存的氨基末端特征是有效体识别的关键.
- 效应因子的跨菌株变异表明在宿主特异性适应中发挥了作用.
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