通过从成熟的B和T细胞供体中转移核而产生的单克隆小鼠
Konrad Hochedlinger1, Rudolf Jaenisch
1Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
Nature
|March 5, 2002
概括
研究人员成功地从成熟的淋巴细胞细胞中克隆小鼠. 这表明,终端分化的细胞可以完全重新编程用于动物克隆,挑战以前的假设.
科学领域:
- 生殖生物学 生殖生物学
- 发育生物学是发展生物学.
- 细胞重新编程的细胞重编程.
背景情况:
- 体细胞克隆是低效的,胚胎死亡率很高.
- 从胚胎干细胞 (ES) 进行克隆更有效,这表明核重编程更容易.
- 之前的假设有利于干细胞而不是分化细胞成功克隆.
研究的目的:
- 研究终端分化的细胞在动物克隆中的潜力.
- 为了证明成熟的淋巴细胞可以被重新编程用于克隆.
- 从差异化的细胞中产生可行的克隆小鼠.
主要方法:
- 采用了经过修改的两步克隆程序.
- 胚胎干细胞是从克隆的胚芽细胞中获得的.
- 这些ES细胞被注射到四平流体胚胎囊中以产生小鼠.
主要成果:
- 单克隆小鼠是由成熟的淋巴细胞通过核转移生成的.
- 从B细胞核克隆的小鼠在所有组织中都显示出可行的免疫球蛋白等位基因.
- 从T细胞核克隆的小鼠在所有组织中都表现出重新排列的T细胞受体基因.
结论:
- 终端分化的细胞可以被重新编程以产生可行的成年克隆动物.
- 这项研究为分化细胞的重编程提供了明确的证据.
- 这些发现挑战了使用干细胞进行高效克隆的必要性.
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