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相关概念视频

Photosystem II01:22

Photosystem II

The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across  two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
Structural Protein Function01:56

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to form...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Structural Protein Function01:56

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to form...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

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相关实验视频

Updated: Jul 5, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

RNA聚合酶II机制:结构照亮了功能.

Nancy A Woychik1, Michael Hampsey

  • 1Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.

Cell
|March 23, 2002
PubMed
概括

细胞的转录依赖于RNA聚合酶II,启动因子和中间体复合体. 结构洞察力,特别是酵母RNA聚合酶II的结构洞察力,揭示了转录机制.

科学领域:

  • 分子生物学分子生物学
  • 结构生物学 结构生物学
  • 生物化学 生物化学

背景情况:

  • 细胞转录是一种基本的生物过程.
  • 关键的参与者包括RNA聚合酶II,启动因子和中间体复合体.
  • 了解转录机制对于细胞功能至关重要.

研究的目的:

  • 阐明真核转录的机制.
  • 为转录因子和复合体提供结构性见解.
  • 专注于酵母RNA聚合酶II的原子分辨率结构.

主要方法:

  • 转录组件的三维结构确定.
  • 高分辨率结构分析.
  • 酵母RNA聚合酶II的结构比较研究.

主要成果:

  • 已获得多个转录因子和复合体的结构数据.
  • 详细的三维结构为分子相互作用提供了洞察力.
  • 已经确定了酵母RNA聚合酶II的原子分辨率结构.

结论:

  • 结构生物学为真核细胞转录提供了关键的见解.

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In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
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In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

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相关实验视频

Last Updated: Jul 5, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

  • 调解器综合体在传输调节信号方面发挥着关键作用.
  • 酵母RNA聚合酶II结构为转录启动提供了一个详细的模型.