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相关概念视频

Transcription01:10

Transcription

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Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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Transcription01:10

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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Transcription01:17

Transcription

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Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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相关实验视频

Updated: Apr 6, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

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将mRNA处理与转录整合在一起.

Nick J Proudfoot1, Andre Furger, Michael J Dye

  • 1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom. nicholas.proudfoot@path.ox.ac.uk

Cell
|March 23, 2002
PubMed
概括
此摘要是机器生成的。

传递 RNA (mRNA) 处理事件,如封闭,拼接和多化发生在转录过程中. 这些过程由转录协调,并相互影响.

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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

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Last Updated: Apr 6, 2026

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科学领域:

  • 分子生物学分子生物学
  • 基因表达 基因表达
  • 处理RNA处理RNA处理

背景情况:

  • 传递 RNA (mRNA) 处理,包括封闭,拼接和多化,是基因表达的关键步骤.
  • 众所周知,这些处理事件发生在基因的转录过程中.
  • 这些反应之间的协调和相互作用尚未完全理解.

研究的目的:

  • 为了阐明mRNA处理的共转录性质.
  • 为了研究封装,拼接和多化之间的协调.
  • 了解RNA聚合酶II在促进这些综合过程中的作用.

主要方法:

  • 对共转录mRNA处理通路的分析.
  • 研究转录和mRNA处理因子之间的分子相互作用.
  • 研究RNA聚合酶II的酸化C终端域 (CTD) 的功能.

主要成果:

  • mRNA的封闭,拼接和多化与转录同时发生.
  • 这些加工反应对它们的效率和特异性具有相互影响.
  • 转录积极协调这些mRNA处理事件.
  • RNA聚合酶II的酸化CTD作为一个平台,与处理因子进行关键的分子相互作用.

结论:

  • mRNA处理是一种集成的配写过程.
  • 转录在协调mRNA封闭,拼接和多基化中发挥着中心作用.
  • RNA聚合酶II的CTD对于将转录与mRNA成熟联系起来至关重要.