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Shaggy/GSK3通过调节Cubitus interruptus来对抗刺的信号传递
Jianhang Jia1, Kazuhito Amanai, Gelin Wang
1Center for Developmental Biology and Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9133, USA.
Nature
|March 26, 2002
概括
皮 (Sgg) 蛋白,一种GSK3激酶,通过促进Ci蛋白处理来负面调节Drosophila中的 Hedgehog路径. 这揭示了Sgg/GSK3在刺信号传输中的新角色.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 香格里拉 (Sgg) 和糖原合成酶激酶3 (GSK3) 是已知的无翼 (Wg) 和Wnt通路的抑制剂.
- 刺 (Hh) 途径对于胚胎发育和模式形成至关重要.
研究的目的:
- 为了调查Shaggy (Sgg) 在Drosophila中的刺 (Hh) 信号通路中的作用.
- 阐明Sgg在Hh通路内调节Cubitus interruptus (Ci) 蛋白的机制.
主要方法:
- 在Drosophila中Sgg功能丧失突变的遗传分析.
- 研究Ci蛋白的加工和酸化状态.
- 对Hh-响应基因表达的分析,包括脑 (dpp) 和wg.
主要成果:
- 失去了sgg功能导致细胞自主积累全长的Ci (Ci155) 和Hh-响应基因的宫外表达.
- Sgg/GSK3,与PKA一起,高酸化,将其向加工成抑制剂形式 (Ci75).
- 刺信号反对Ci蛋白解,可能是通过促进其脱.
结论:
- Shaggy (Sgg) /GSK3作为Drosophila. Hedgehog中子通路的负调节者.
- Sgg/GSK3和PKA合作控制Ci蛋白处理,这是Hh信号传导中的一个关键事件.
- 刺信号调节的酸化状态,以调节通路活动.
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