在芽酵母中转录调节的遗传剖析
Rachel B Brem1, Gaël Yvert, Rebecca Clinton
1Fred Hutchinson Cancer Research Center (FHCRC), 1100 Fairview Avenue North, D4-100, Seattle, WA 98109, USA and Howard Hughes Medical Institute.
概括
在酵母中研究了基因表达的遗传变异. 研究人员确定了影响基因表达模式的cis-和trans-acting遗传位置,揭示了复杂的遗传.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 了解基因表达自然变异的遗传基础至关重要.
- 麦芽菌 (Saccharomyces cerevisiae) 作为研究基因调节的模型生物.
研究的目的:
- 研究基因表达模式中的自然变异的遗传结构.
- 为了确定调节酵母杂交中的基因表达的基因位点.
主要方法:
- 对实验室和野生菌株Saccharomyces cerevisiae之间的交叉进行了遗传链接分析.
- 分析了全基因组表达模式,以确定差异表达的基因.
- 定量特征位点 (QTL) 映射用于检测基因表达的遗传调节器.
主要成果:
- 超过1500个基因在母酵母菌株之间表现出差异性表达.
- 发现570个基因的表达水平与一个或多个遗传位置有关.
- 大多数表达水平显示复杂的遗传模式.
- 确定了两个类别的基因位点:cis-acting (调节单个基因) 和 trans-acting (调节多个基因).
- 确定了8个转基因作用位点,每个位点都影响了7至94个功能相关基因的表达.
结论:
- 酵母中基因表达的自然变异受到cis和trans作用的遗传因素的影响.
- 转基因作用位置在协调功能相关基因组的表达方面发挥着重要作用.
- 这项研究提供了对基因表达多样性的基因架构的洞察.
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