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在活老鼠中对心脏记者基因表达的光学成像

Joseph C Wu¹, Masayuki Inubushi, Gobalakrishnan Sundaresan

¹Crump Institute for Molecular Imaging and Department of Medicine, Division of Cardiology, UCLA School of Medicine, Los Angeles, Calif, USA.

|April 10, 2002

概括

这项研究显示,使用火虫光酶在老鼠心脏基因表达的非侵入性成像. 这种方法允许追踪基因疗法对活体的长期影响,提高研究能力.

科学领域:

生物医学成像学生物医学成像学
分子生物学分子生物学
基因治疗是一种基因疗法.

背景情况:

心脏基因转移研究传统上依赖于侵入性死后分析.
非侵入性成像为随着时间的推移在体内跟踪基因表达提供了优势.

研究的目的:

为了证明心脏记者基因表达在活老鼠中的非侵入性成像的可行性.
建立一种评估心脏基因转移时间和空间特征的方法.

主要方法:

鼠接受了腺病毒表达火虫光酶 (Ad-CMV-Fluc) 的直接心肌内射.
生物发光成像使用冷却的CCD摄像机在D-路西费林后进行.
报告者基因活性被量化为每分钟相对光单位 (RLU/min).

主要成果:

心脏光酶活性在转导后的14天内下降.
图像检测方法表现出对Ad-CMV-Fluc.c.形成斑块的1x10(7) 单位的敏感性.
在体内成像信号与体内光酶活性有很强的相关性 (r2=0.92).

结论:

这项研究成功地证明了活老鼠心脏记者基因表达的非侵入性成像.
开发的方法允许实时评估基因表达位置,大小和持续时间.
这种技术可以显著帮助未来的心脏基因治疗研究.

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