用一种芳香醇更快地折叠含有二硫化物的蛋白质
Jonathan D Gough1, Rhondye H Williams, Anthony E Donofrio
1Department of Chemistry, Syracuse University, Syracuse, New York 13244, USA.
Journal of the American Chemical Society
|April 11, 2002
概括
研究人员通过用一种新型芳香醇,4-mercaptobenzeneacetate,替换谷氨来提高蛋白质折叠率. 这种新方法为含有二硫化物的蛋白质,如RNase A.提供了5-6倍的折叠速度.
科学领域:
- 生物化学 生物化学
- 蛋白质化学 蛋白质化学
- 化学生物学 化学生物学
背景情况:
- 含有二硫化物蛋白质的体外蛋白质折叠通常是缓慢的.
- 传统的方法使用一种谷氨/谷氨二硫化物氧化还原缓冲器.
研究的目的:
- 为了提高体外蛋白质折叠的速度.
- 为了研究蛋白质折叠过程,使用一种新型芳香醇.
主要方法:
- 用4-mercaptobenzeneacetate (芳香醇1) 取代了谷氨.
- 在pH值7.0和7.7.7下研究了减少和杂乱的RNase A的折叠.
- 同时确定最佳的硫醇度和测量折叠率.
主要成果:
- 与谷氨相比,芳香醇4-甲乙酸增加了5-6倍的折叠率.
- 折叠率对于减少和混的RNase A.都是相似的.
- 折叠率的增强显示了pH的最小变化 (7.0对7.7).
结论:
- 芳香醇4-甲乙酸显著加速体外蛋白质折叠.
- 这种硫醇为蛋白质折叠研究提供了比传统的氧化还原缓冲器更有效的替代品.
- 这些发现提供了关于蛋白质折叠过程中二硫化物键的形成的见解.
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