酵母酵母中的2'-氧氧醇,是维生素B6和维生素B1的生物合成前体
Johannes Zeidler1, Nisar Ullah, Ram Nath Gupta
1Department of Chemistry, McMaster University, Hamilton, Ontario, Canada L8S 4M1.
Journal of the American Chemical Society
|April 25, 2002
概括
这项研究表明,2'-hydroxypyridoxol是酵母从糖中生产维生素B6的关键中间体. 它还证实了pyridoxol在形成胺 (维生素B1) 的pyrimidine部分中的作用.
科学领域:
- 生物化学 生物化学
- 酵母的新陈代谢 酵母的新陈代谢
- 维生素合成 维生素合成
背景情况:
- 维生素B6和胺 (维生素B1) 是必需的营养素.
- 了解酵母中这些维生素的生物合成途径对于代谢工程和营养研究至关重要.
- 以前的研究表明,pyridoxol是前体,但中间步骤尚未完全阐明.
研究的目的:
- 为了研究2 - - 氧二醇在Saccharomyces cerevisiae中的维生素B6生物合成中的作用.
- 为了确认胺合成中的胺基单元的起源.
主要方法:
- 培养Saccharomyces cerevisiae与专门标有的2 -hydroxypyridoxol进行培养.
- 隔离皮里多克萨二化物和硫胺化物化物.
- 使用2H核磁共振 (NMR) 光谱分析分离的化合物.
主要成果:
- 来自标记为2 - 氧二醇的在氧胺和胺的胺环中被检测到.
- 特定的标签模式证实了在预测的地点加入.
- 这提供了直接证据,证明2 - 氧二醇是维生素B6形成中的中间体.
结论:
- 2 - 基二醇无疑是酵母从C5糖到维生素B6的途径中的中间体.
- 皮里多克索尔作为胺的皮里米丁单元的前体,为完整的C5N片段做出了贡献.
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