在体外选择ATP结合受体使用核糖核复合物
Takashi Morii1, Masaki Hagihara, Shin-ichi Sato
1Institute of Advanced Energy, Kyoto University, and PRESTO, Japan Science and Technology Corporation, Uji, Kyoto 611-0011, Japan. t-morii@iae.kyoto-u.ac.jp
Journal of the American Chemical Society
|April 25, 2002
概括
研究人员使用和RNA支架设计了一种新的ATP核糖核受体. 这个人工受体是人造受体.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 结构生物学 结构生物学
背景情况:
- 核糖体展示了RNA-蛋白质复合体的多样性结构.
- 人工受体对于分子识别和药物设计至关重要.
研究的目的:
- 使用-RNA复合体设计一种新型的人造受体.
- 创建一个特定的核糖核受体,用于腺三酸盐 (ATP).
主要方法:
- 设计了一个支架,将短和RNA与随机核酸区域结合起来.
- 利用HIV-1 Rev反应元件用于核糖核池的形成.
- 在体外选择中用于分离具有特定结合性质的RNA寡核酸.
主要成果:
- 成功生成了具有高ATP特异性的核糖核受体.
- 鉴定出的ATP结合的核糖核序列与已知的ATP体有所不同.
- ATP结合依赖于Rev的存在,并通过其氨基酸替代物调节.
结论:
- 类成分是RNA受体功能结构的组成部分.
- 的RNA结合区域外的氨基酸会影响ATP结合.
- 这种方法为设计定制的核酸受体和酶提供了一种策略.
相关概念视频
Secondary Active Transport
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
Secondary Active Transport
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
ATP Energy Storage and Release
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
One example of energy coupling using ATP involves a...
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Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
ATP Energy Storage and Release
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
One example of energy coupling using ATP involves a...
Secondary Active Transport
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...


