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脊椎动物蛋中细胞静止因子的停止需要Emi1
Julie D R Reimann1, Peter K Jackson
1Department of Pathology, Stanford University School of Medicine, Stanford, California 94305-5324, USA.
Nature
|April 27, 2002
概括
胚胎发育依赖于细胞静止因子 (CSF) 来阻止脊椎动物的卵在介质变异II. 研究人员发现,Emi1蛋白对于这种停止至关重要,作为CSF活动的关键调解者.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 脊椎动物的卵在半分裂II时停止,这是由于稳定的环林B和高的环林B/Cdc2激酶活性.
- 细胞静止因子 (CSF) 抑制了亚纳相促进复合体/环体 (APC),防止卵子中的甲相退出.
- 受精触发了的增加,使中枢神经液失活,激活APC,并导致循环蛋白B的破坏和线粒体的退出.
研究的目的:
- 研究Emi1在脊椎动物卵的CSF捕获机制中的作用.
- 确定 Emi1 是否是 CSF 活动的关键调解者.
主要方法:
- 来自CSF提取物的Emi1的免疫减弱.
- 评估含有和不含的Emi1-贫提取物中的B环素降解和线粒脱出.
- 评估将Emi1重新添加到耗尽的提取物的影响.
主要成果:
- 艾米1是抑制APC的必要和足够的,并防止CSF捕获的卵子中的线粒退出.
- 缺乏Emi1的脑液提取物降解环林B,在没有的情况下过早退出线索.
- 将Emi1添加到耗尽的提取物中,可以恢复CSF停止状态.
结论:
- 对于未受精的脊椎动物卵的第二阶段停止,Emi1是必需的.
- 艾米1作为细胞静止因子 (CSF) 活动的长期寻求的调解者,维持中介性停止.
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