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Plotting of Topographic Maps

Plotting of Topographic Maps

Topographic maps represent the Earth's surface features using contour lines, which connect points of equal elevation to create a two-dimensional representation of three-dimensional terrain. Creating a topographic map requires a systematic approach.Begin by plotting a scaled grid and marking intersections corresponding to the survey's elevation data points. Assign elevation values at these intersections to build the base map. Next, determine contour levels using a consistent contour interval,...

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Updated: Jul 7, 2026

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

Published on: July 5, 2017

绘制细胞染色体C折叠景观的地图.

Julia G Lyubovitsky¹, Harry B Gray, Jay R Winkler

¹Beckman Institute, California Institute of Technology, Pasadena, California 91125, USA.

Journal of the American Chemical Society

|May 9, 2002

概括

研究人员使用光能量转移绘制了酵母iso-1细胞染色体c的蛋白质折叠能量格局. 他们确定了不同的崩和扩展的多种群,这些种群迅速寻找本地结构.

科学领域:

生物化学生物化学
生物物理学的生物物理.
分子生物学分子生物学

背景情况:

蛋白质折叠是一个由构造性能量格局所支配的基本过程.
了解这种景观对于破译蛋白质结构和功能至关重要.

研究的目的:

为了绘制Saccharomyces cerevisiae iso-1 cytochrome c.的折叠能量格局.
为了研究蛋白质折叠中间体的动态.

主要方法:

利用光能量转移 (FRET) 动力学来监测折叠.
在折叠过程中测量了C端光体和血群之间的距离.
分析了不同折叠阶段的蛋白质种群.

主要成果:

在重新折叠1毫秒内确定了两个不同的,快速平衡的群体:崩的结构 (平均距离27 Å) 和扩展的多 (>50 Å).
原生折叠形成发生在大约300毫秒的时间尺度上,由血结合调节.
折叠的景观有一个狭窄的中央漏斗,上面有一个平坦的边缘,促进中间体的快速相互转换.

结论:

细胞染色体c的折叠路径涉及崩和扩展状态之间的快速平衡.

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血红蛋白结合在蛋白质折叠的最后阶段起着至关重要的作用.

经过实验确定的景观为蛋白质折叠的机制提供了洞察力.