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Updated: Jul 18, 2026

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

在实验室中以蛋白质为媒介合成GeranylGeranylated Rab7蛋白质

Kirill Alexandrov1, Ines Heinemann, Thomas Durek

  • 1Max-Planck-Institut für Molekulare Physiologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany. kirill.alexandrov@mpi-dortmund.mpg.de

Journal of the American Chemical Society
|May 16, 2002
PubMed
概括

研究人员开发了一种新的方法来制造半合成脂质蛋白,特别是单烯酸Rab7 GTPase,使用体外蛋白质结合. 这一突破使得用于生物技术和生命科学研究的改性蛋白质的生产成为可能.

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Rab Proteins01:14

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...