TFIIH在RNA聚合酶I转录中起着至关重要的作用
Sebastian Iben1, Herbert Tschochner, Mirko Bier
1Division of Molecular Biology of the Cell II, German Cancer Research Center, D-69120, Heidelberg, Germany.
Cell
|May 23, 2002
概括
转录因子II H (TFIIH) 复合体对于核糖体RNA合成至关重要. 这项研究揭示了TFIIH.
科学领域:
- 分子生物学分子生物学
- 基因表达 基因表达
- 修复DNA修复DNA的修复
背景情况:
- 转录因子II H (TFIIH) 是一种关键的蛋白质复合体,参与DNA修复和蛋白质编码基因转录.
- 它在核糖体RNA (rRNA) 合成中的作用以前尚未确定.
研究的目的:
- 研究TFIIH在核糖体RNA合成中的作用.
- 为了确定是否需要TFIIH用于RNA聚合酶I转录.
主要方法:
- 使用了TFIIH中温度敏感突变的酵母菌株.
- 使用小鼠细胞分数进行了体外转录试验.
- 研究了TFIIH在细胞核中的定位及其与RNA聚合酶I和TIF-IB的关联.
主要成果:
- 在酵母TFIIH突变菌株中,前rRNA合成受损.
- 发现TFIIH定位在细胞核中,并在小鼠细胞中与RNA聚合酶I和TIF-IB结合.
- 缺少TFIIH的转录系统是不活跃的,并且添加TFIIH恢复了活动.
- TFIIH是生产性的,但不是堕胎的,rDNA转录所需的,这表明它具有启动后的功能.
结论:
- 在体内和体外,TFIIH对于核糖体RNA合成至关重要.
- TFIIH在RNA聚合酶I转录中起着启动后的作用.
- 这项工作建立了rRNA基因转录和转录合修复之间的分子联系.
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