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诱导和抑制RNA沉默由动物病毒的诱导和抑制
Hongwei Li1, Wan Xiang Li, Shou Wei Ding
1Department of Plant Pathology and Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA.
概括
RNA沉默,一种防御机制,针对Drosophila的群体房子病毒 (FHV). 编码FHV的蛋白B2抑制了这种抗病毒防御,这表明植物和动物之间存在着保存的途径.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 遗传学 是一个遗传学.
背景情况:
- RNA沉默是一种在植物和动物中发现的保存序列特定RNA降解过程.
- 这种机制在基因调节和对外来核酸的防御中起着至关重要的作用.
研究的目的:
- 为了研究RNA沉默在Drosophila宿主细胞中的作用,在群体病毒 (FHV) 感染期间.
- 为了确定FHV是否与RNA沉默机制相互作用并可能逃避.
- 为了确定参与抑制RNA沉默的病毒因素.
主要方法:
- 德洛索菲拉黑虫 (Drosophila melanogaster) 感染了羊群病毒 (FHV).
- 分析FHV复制及其与宿主RNA沉默通路的相互作用.
- 在宿主细胞和转基因植物中表达FHV编码的蛋白质,特别是B2,以评估它们对RNA沉默的影响.
主要成果:
- 群屋病毒 (FHV) 已被证明是Drosophila.的RNA沉默的发起者和目标.
- FHV感染需要抑制RNA沉默,由病毒蛋白B2介导.
- 在转基因植物中,FHV编码的蛋白B2证明了抑制转基因植物RNA沉默的能力.
结论:
- RNA沉默功能作为动物细胞中的自适应性抗病毒防御机制.
- 在感染期间,FHV编码的蛋白B2对于克服宿主RNA沉默反应至关重要.
- 在抑制跨物种RNA沉默方面B2的保存功能提供了植物和动物共享RNA沉默途径的证据.
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