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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

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Published on: November 28, 2017

通过模块化全有机合成策略制备全跨均 (13) C标记的视网膜. 有机合成对结构和功能研究的新兴中心贡献,在蛋白质研究中具有原子分辨率.

Alain F L Creemers¹, Johan Lugtenburg

¹Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.

Journal of the American Chemical Society

|May 30, 2002

概括

均的碳-13标记的全透视网膜用一种新的模块化有机战略合成. 这种方法实现了高同位素丰富,并允许对视网和胡卜素进行生物研究的多功能标签.

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科学领域:

有机合成有机合成
同位素标记的标记
生物化学生物化学

背景情况:

全透视网膜是生物系统中,特别是视觉系统中至关重要的分子.
稳定同位素标记对于NMR光谱等先进分析技术至关重要.
之前合成标记网类药物的方法在范围和效率上是有限的.

研究的目的:

开发一种通用和高效的方法来合成标记为全跨视网膜的均碳-13的合成.
创建一个模块化策略,使各种同位素和衍生物的合成.
为研究生物系统提供高分辨率的工具.

主要方法:

采用了融合模块化全有机合成策略.
合成了四个关键的 (13) C 标记模块:一种酸,一种乙烯酸乙酸,一种甲基,一种环烯碳烯.
这些模块被组合起来,构建了标有统一标签的全跨视网膜.

主要成果:

均标记[(13) C(20) 的全跨视网膜被制备了>99%的同位素合并.
模块化方法允许合成所有同位素和化学修饰的视网蛋白.
该策略可适应创建其他稳定的同位素标记生物大分子.

结论:

已经建立了一个强大而灵活的模块化合成,用于统一标记的全跨视网膜.
这种方法促进了各种 (13) C标记的视网和胡卜素的生产.
合成的化合物是生物系统和蛋白质的高分辨率研究的宝贵工具.