相关实验视频
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Microinjection of Medaka Embryos for use as a Model Genetic Organism
Published on: December 22, 2010
DMY是一种Y特异的DM域基因,在Medaka鱼中,它是男性发育所需的基因
Masaru Matsuda1, Yoshitaka Nagahama, Ai Shinomiya
1Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Nature
|May 31, 2002
概括
研究人员确定了DMY基因对于梅达卡鱼的男性性别决定至关重要. 在DMY的突变导致XY鱼发展为雌性,突出其在丸发育中的作用.
科学领域:
- 遗传学 是一个遗传学.
- 发育生物学 发展生物学
- 进行比较基因组学.
背景情况:
- 确定性别的基因Sry在哺乳动物中已知,但其在非哺乳动物脊椎动物中的对应基因仍然难以捉摸.
- 了解不同物种的性别决定机制是进化和遗传研究的关键.
研究的目的:
- 为了识别梅达卡鱼 (Oryzias latipes) 中的性别决定基因.
- 研究候选基因在丸发育中的功能.
主要方法:
- 用复合断点分析和删除分析来缩小Y染色体上的性别决定区域.
- 枪支测序确定了这一关键区域内的基因.
- 对候选基因进行基因表达分析和突变研究.
主要成果:
- 确定性别的区域定位在Y染色体的250 kb段.
- 在这个区域预测了27个基因,其中3个在性差异化过程中表达.
- 确定了Y特异性基因DMY (PG17),并证明它对男性发育至关重要,因为突变导致XY雌性.
结论:
- DMY是一种Y特异性基因,对梅达卡鱼的丸发育至关重要.
- DMY是医学界决定性基因的主要候选者.
- 这一发现揭示了脊椎动物性决定的演变.
相关概念视频
Pedigree Analysis
Overview
Sex-linked Disorders
Like autosomes, sex chromosomes contain a variety of genes necessary for normal body function. When a mutation in one of these genes results in biological deficits, the disorder is considered sex-linked.
X and Y Chromosomes
Among mammals, the gender of an organism is determined by the sex chromosomes. Humans have two sex chromosomes, X and Y. Every human diploid cell has 22 pairs of autosomes and one pair of sex chromosomes. A human female has two X chromosomes, while a male has one X chromosome and one Y chromosome.
The germline cells such as egg and sperm cells carry only half the number of chromosomes, i.e., 22 autosomes and one sex chromosome. All eggs have an X chromosome, while sperm cells can carry an X or...
The germline cells such as egg and sperm cells carry only half the number of chromosomes, i.e., 22 autosomes and one sex chromosome. All eggs have an X chromosome, while sperm cells can carry an X or...
The Y Chromosome Determines Maleness
The Y chromosome is a sex chromosome found in several vertebrates and mammals, including humans. In addition to 22 pairs of autosomes, the human males have one X chromosome and one Y chromosome. In these organisms, the presence or absence of the Y chromosome determines the development of male traits.
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size. Today,...
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size. Today,...
The Ratio of X Chromosome to Autosomes
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Dosage Compensation
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...

