转录协激活剂TRAP220是PPAR马2刺激脂肪生成所需的
Kai Ge1, Mohamed Guermah, Chao-Xing Yuan
1Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Nature
|May 31, 2002
概括
TRAP220蛋白对于脂肪生成至关重要,在TRAP复合体内作为PPAR玛(2) -选择性协活性剂. 缺少它会阻断脂肪细胞的分化,但不会阻断肌肉细胞的发展.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 甲状腺激素受体关联蛋白 (TRAP) 复合体,也称为调解器,是一种转录协活性剂.
- TRAP220是TRAP复合体的一个子单元,与核受体相互作用.
- 过氧体增殖器激活受体玛2 (PPAR玛2)) 对于脂肪生成至关重要.
研究的目的:
- 为了研究TRAP220在PPAR玛(2) 介导的脂肪生成中的作用.
- 为了确定TRAP220的功能是否特定于某些分化途径.
主要方法:
- 来自Trap220淘汰赛小鼠的纤维细胞培养物.
- 刺激脂肪生成和肌形成.
- 对基因表达和蛋白质与蛋白质相互作用的分析.
- 在体外同活性剂测定.
主要成果:
- 陷220(-/-) 纤维细胞未能经历PPAR玛(2) 刺激的脂肪生成.
- 在Trap220(-/-) 纤维细胞中,肌体发生没有受到影响.
- 在体外,TRAP220直接协同激活PPAR玛(2) 转录.
- 这种TRAP220-PPAR gamma(2) 相互作用是依赖于连接体的.
结论:
- 通过TRAP复合体,TRAP220充当PPAR玛(2) 选择性协活性剂.
- 在纤维细胞中,TRAP220对于脂肪生成是必不可少的,但对纤维细胞中的肌体生成不是必不可少的.
- 这凸显了TRAP220在调节不同细胞分化途径方面的特殊作用.
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