相关实验视频
Updated: May 23, 2026
09:41
Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
Published on: June 17, 2014
克雷门蛋白是调节Wnt/β-catenin信号传递的Dickkopf受体
Bingyu Mao1, Wei Wu, Gary Davidson
1Molecular Embryology Division, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
Nature
|June 7, 2002
概括
克雷门1和克雷门2蛋白作为Dikkkopf1 (Dkk1) 的受体,抑制Wnt/β-catenin信号传递. 这些克雷门蛋白与Dkk1一起调节LRP6 Wnt受体,影响细胞生长和分化.
科学领域:
- 分子生物学分子生物学
- 发育生物学 发展生物学
- 细胞信号传递 细胞信号传递
背景情况:
- Wnt信号传递对于细胞生长和分化至关重要.
- 规范性Wnt信号传递涉及通过Frizzled和LRP5/6受体稳定β-catenin.
- Dickkopf1 (Dkk1) 通过结合LRP5/6.1来抑制Wnt信号传递.
研究的目的:
- 为了确定Dickkopf1 (Dkk1) 的新型受体.
- 研究克雷门1和克雷门2在Wnt/β-catenin信号传递中的作用.
- 阐明Dkk1和克雷门蛋白抑制Wnt信号传递的机制.
主要方法:
- 同免疫沉测试检测蛋白质复合体.
- 基于细胞的测试来测量Wnt/β-catenin信号活动.
- 孔焦显微镜可视化受体局部化和内细胞化.
主要成果:
- 克雷门1和克雷门2被确定为高亲和度Dkk1受体.
- 克雷门蛋白与Dkk1合作,抑制Wnt/β-catenin信号传递.
- 克雷门2与Dkk1和LRP6形成三元复合体,促进LRP6的内细胞化.
结论:
- 克雷门1和克雷门2是Dkk1介导的Wnt信号的抑制复合物的关键组成部分.
- 这个复合体通过调节等离子体膜LRP6受体水平来调节正规的Wnt信号.
- 这些发现为脊椎动物中Wnt信号的调节提供了新的见解.
相关概念视频
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...