相关实验视频
Updated: Jul 20, 2026

14:20
Absolute Quantum Yield Measurement of Powder Samples
Published on: May 12, 2012
在对Jarzynski等式的实验测试中,从非平衡测量中获得平衡信息
Jan Liphardt1, Sophie Dumont, Steven B Smith
1Department of Chemistry, Biophysics Graduate Group, University of California, Berkeley, CA 94720, USA.
概括
研究人员对Jarzynski进行了测试.
科学领域:
- 热力学是一种热力学.
- 统计力学 统计力学
- 单分子生物物理学 单分子生物物理学
背景情况:
- 贾辛斯基的平等 (1997年) 将不可逆转的工作与平衡的自由能量差异联系起来.
- 这使得平衡参数可以从非平衡过程中导出.
- 实验热力学的一个根本挑战.
研究的目的:
- 为了实验验证Jarzynski的平等.
- 为了弥合平衡和非平衡系统的统计力学.
- 扩展单分子操纵数据的热力学分析.
主要方法:
- 机械拉伸一个单独的RNA分子.
- 执行可逆和不可逆的拉伸协议.
- 将Jarzynski的等式应用于不可逆转的工作轨迹.
主要成果:
- 贾辛斯基的等式准确地恢复了自由能量差异 (DeltaG).
- 结果在可逆拉伸的平均工作的k(B) T/2以内.
- 第一次实验实施和测试Jarzynski的平等.
结论:
- 贾辛斯基等式在平衡和不平衡的统计力学之间提供了一座桥梁.
- 能够对超出平衡条件的单分子实验进行热力学分析.
- 在生物物理背景下验证理论预测.
相关概念视频
Uncertainty in Measurement: Reading Instruments
Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
Static Equilibrium - II
Static equilibrium is a special case in mechanics that is very important in everyday life. It occurs when the net force and the net torque on an object or system are both zero. This means that both the linear and angular accelerations are zero. Thus, the object is at rest, or its center of mass is moving at a constant velocity. However, this does not mean that no forces are acting on the object within the system. In fact, there are very few scenarios on Earth in which no forces are acting upon...
Rigid Body Equilibrium Problems - II
A rigid body is in static equilibrium when the net force and the net torque acting on the system are equal to zero.
Consider two children sitting on a seesaw, which has negligible mass. The first child has a mass (m1) of 26 kg and sits at point A, which is 1.6 meters (r1) from the pivot point B; the second child has a mass (m2) of 32 kg and sits at point C. How far from the pivot point B should the second child sit (r2) to balance the seesaw?
Consider two children sitting on a seesaw, which has negligible mass. The first child has a mass (m1) of 26 kg and sits at point A, which is 1.6 meters (r1) from the pivot point B; the second child has a mass (m2) of 32 kg and sits at point C. How far from the pivot point B should the second child sit (r2) to balance the seesaw?
Wald-Wolfowitz Runs Test II
The Wald-Wolfowitz runs test, commonly referred to as the runs test, is a nonparametric test used to assess the randomness of ordered data. The test evaluates the number of runs, which are consecutive sequences of similar elements within the data. If the number of runs is significantly higher or lower than expected, the data is considered non-random, indicating a detectable pattern or structure.
For binary data, runs are identified using symbols such as + and −, or equivalently, 1s and 0s. In...
For binary data, runs are identified using symbols such as + and −, or equivalently, 1s and 0s. In...
Instrument Calibration
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
Measurement of Air Content in Concrete
Air content measurement in concrete is critical for ensuring structural integrity and durability of concrete structures, especially in environments prone to severe weather conditions. Accurate air content analysis optimizes concrete's resistance to freeze-thaw cycles and enhances its workability and strength. Several methods are standardized under ASTM guidelines to measure the air content in fresh concrete, each suitable for different concrete types and conditions.
The pressure method,...
The pressure method,...

