氨基酸-tRNA合成由树脂固定的 ribozyme 树脂
Hiroshi Murakami1, Neil J Bonzagni, Hiroaki Suga
1Department of Chemistry, University at Buffalo, State University of New York, Buffalo, New York 14260-3000.
Journal of the American Chemical Society
|June 13, 2002
概括
研究人员开发了一种新的树脂固定式 рибо酶方法,用于转移RNA (tRNA) 的氨基酸化. 这种实用技术有效地为体外翻译系统提供非自然氨基酸tRNA.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 合成生物学 合成生物学
背景情况:
- 转移RNA (tRNA) 的氨基化对于蛋白质合成至关重要.
- 对于先进的体外翻译系统,需要有效地产生非天然氨基tRNA.
研究的目的:
- 为tRNA氨基化开发一种新且实用的方法.
- 在氨基化反应中重复使用的 ribozyme 固定.
- 供应各种非天然的氨基酸tRNA用于体外翻译.
主要方法:
- 通过共价链接将氧化 ribozyme 固定在水树脂上.
- 使用 ribozyme-resin 列和一种甲基 (CME) 氨基酸基质的tRNA 的氨基化.
- 在多个氨基化循环中回收 ribozyme-resin 列.
主要成果:
- 通过使用固定式 ribozyme,成功实现了 tRNA 的氨基化.
- 里波酶树脂列表现出稳定性,并且在最小的活动损失的情况下回收了多达5次.
- 该方法有效地产生了氨基酸-tRNAs.
结论:
- 建立了一种新的,实用的基于 ribozyme 的氨基化方法.
- 固定式利博酶系统为tRNA氨基化提供了稳定和可回收的方法.
- 这种技术具有很大的潜力,可以为在体外翻译提供各种非天然氨基酸tRNA.
相关概念视频
02:43Nucleic acids
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
02:43Nucleic Acids
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
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Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
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02:36Transfer RNA Synthesis
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
02:26tRNA Activation
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...