节节酸A的生物合成:前体研究
Kevin M Byrne1, Scott K Smith, John G Ondeyka
1Merck Research Laboratories, P.O. Box 2000, Rahway, New Jersey 07065-0900, USA. kevin_byrne@merck.com
Journal of the American Chemical Society
|June 13, 2002
概括
诺杜利斯酸 在诺杜利斯菌中的生物合成涉及美酸路径. 与预期相反,托芬不是直接的前体;相反,安德拉尼酸和核糖被纳入了这个甲.
科学领域:
- 自然产品生物合成 自然产品生物合成
- 菌类学 菌类学是指菌类学.
- 有机化学 有机化学
背景情况:
- 诺杜利斯酸A (NAA) 是一种来自诺杜利斯菌的印二烯天然产物.
- 在结构上,NAA与帕斯巴林代谢产物有关.
- 拟议的生物合成涉及酸/美酸路径和geranylgeranyl pyrophosphate (GGPP) 与酸盐的凝结.
研究的目的:
- 为了阐明诺杜利斯酸A (NAA) 的生物合成途径.
- 调查梅瓦龙酸和托前体在NAA形成中的作用.
- 为了识别用于二生物合成的新型前体.
主要方法:
- 使用洗细胞程序使用突变的诺杜利斯培养物 (MF6244).
- 包括标记的前体:2-(13) 酸,2-(13) 甲, (14) 和 (13) 三, (14) , (13) ,和 (15) N-氨酸,和 (14) 和 (13) 糖.
- 分析了纳入模式,以确定生物合成路径.
主要成果:
- 确认了NAA东侧的经典美酸模式.
- 观察到三个额外的异化形成西部和南部地区.
- 没有发现标记的托芬,这与之前的假设相矛盾.
- 在NAA中显著地纳入了人酸和核糖.
结论:
- 介绍了诺杜利斯酸A生物合成的新途径.
- 确定NAA生物合成偏离了拟议的托凝结.
- 在经过修订的生物合成模型中,鉴定了人酸和核糖作为关键前体.
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