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发芽酵母中的染色质边界:核孔连接的核孔连接
Kojiro Ishii1, Ghislaine Arib, Clayton Lin
1Departments of Biochemistry and Molecular Biology, University of Geneva, 30, Quai Ernest-Ansermet, Geneva, Switzerland.
Cell
|June 14, 2002
概括
核运输蛋白质作为酵母中的染色质边界元素,将基因与核孔综合体结合起来,以防止异性染色质的传播和维持基因表达.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 酵母遗传学 酵母遗传学
背景情况:
- 染色体边界活动 (BAs) 定义了将基因隔离于沉默的区域.
- 在Saccharomyces cerevisiae中沉默的交配类型的位置HML是研究异色素蛋白形成和边界元素的模型.
- 参与核运输的蛋白质越来越多地被认为具有超出其规范功能的作用.
研究的目的:
- 在Saccharomyces cerevisiae中识别和描述新的染色质边界活动.
- 研究核细胞质运输蛋白在表观遗传调节中的作用.
- 阐明运输蛋白在HML位点建立边界活动的机制.
主要方法:
- 基因查以确定边界活动.
- 免疫定位和活细胞成像以追踪蛋白质定位.
- 染色体免疫沉 (ChIP) 来评估DNA-蛋白相互作用.
- 基因操纵包括基因删除和向的DNA元素修饰.
主要成果:
- 一些核细胞质运输蛋白,包括出口素Cse1p,Mex67p和Los1p,表现出色素边界活性.
- 这些运输蛋白在物理上将HML位点与核孔综合体 (NPC) 的Nup2p受体结合在一起.
- 删除NUP2消除了这些运输蛋白的边界活性,而对DNA的有针对性的绑定恢复了它.
结论:
- 通过核运输蛋白质将基因组位点物理连接到NPC,可以建立染色体边界.
- 这种机制可以防止异色染色素的扩散,从而保持活跃的转录状态.
- 核孔复合组件在基因表达的表观遗传调节中发挥着直接作用.
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