两个不同的Pseudomonas效应蛋白与Pto激酶相互作用并激活植物免疫力
Young Jin Kim1, Nai Chun Lin, Gregory B Martin
1Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA.
Cell
|June 14, 2002
概括
茄子 番茄子 番茄子
科学领域:
- 植物病理学 植物病理学
- 分子植物微生物相互作用.
- 植物免疫力 植物免疫力
背景情况:
- 番茄Pto胺/氨酸激酶对抗表达AvrPto.的Pseudomonas注射器菌株的耐药性至关重要.
- 在激活植物防御机制时,Pto-AvrPto相互作用至关重要.
研究的目的:
- 识别和描述与番茄Pto kinase相互作用的新型细菌效应体.
- 阐明这些效应因子调节植物免疫力的机制.
主要方法:
- 蛋白质与蛋白质相互作用测试以确认Pto与细菌效应体之间的结合.
- 对细菌III型分泌系统进行分析,以提供效应器.
- 基因分析,以确定有效体-Pto相互作用的关键氨基酸残留物.
主要成果:
- 鉴定出第二种 Pseudomonas 效应体,即 AvrPtoB,该效应体与番茄Pto 激酶特别相互作用.
- 通过III型分泌系统,AvrPtoB进入植物细胞,并触发了Pto-依赖的防御系统.
- 尽管序列相似性很低,但AvrPtoB利用与AvrPto类似的氨基酸残留物进行Pto相互作用.
结论:
- 两个不同的细菌效应剂,AvrPto和AvrPtoB,通过与相同的宿主蛋白激酶Pto相互作用来激活番茄免疫力.
- 这些效应器采用类似的结构机制来参与Pto,突出显示了病原体毒性中保存的策略.
- 这些发现扩大了我们对植物免疫受体识别和细菌效应器功能在植物疾病 (如斑点病) 的理解.
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