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异色素组合素H3甲基化模式对阿拉比多普西斯基因DDM1的依赖性

Anne-Valérie Gendrel¹, Zachary Lippman, Cristy Yordan

¹Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Science (New York, N.Y.)

|June 22, 2002

概括

阿拉比多普西斯基因DDM1 (减少DNA甲基化1) 维持DNA甲基化和基因沉默. 它还调节了基因组甲基化模式,表明基因组修饰指导了植物中的DNA甲基化.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
植物分子生物学植物分子生物学
染色体生物学染色体生物学

背景情况:

阿拉比多普西斯基因DDM1 (减少DNA甲基化1) 对于维持DNA甲基化和沉默转子和转基因至关重要.
DDM1与SWI/SNF染色体重塑基因的相似性表明,DDM1在DNA甲基化中的间接作用.

研究的目的:

调查DDM1在维持基因素甲基化模式中的作用.
为了阐明DDM1,基因组甲基化和DNA甲基化在Arabidopsis heterochromatin之间的关系.

主要方法:

在野生类型和ddm1突变植物Arabidopsis heterochromatin中分析基因组H3甲基化模式 (lysine 4和lysine 9).
评估DNA甲基化水平与基因素修饰变化的结合.

主要成果:

DDM1对于维持基因素H3甲基化模式,特别是H3K9甲基化在 heterochromatin 中至关重要.
DDM1功能的丧失导致DNA甲基化降低,并从H3K9甲基化转向H3K4甲基化.
在ddm1突变体中的转子和静态基因显示了改变的基因组甲基化概况.

结论:

基因组H3甲基化模式,特别是H3K9甲基化,可能引导植物基因组中转子体的DNA甲基化.
基因组甲基化模式的改变可以导致低甲基化DNA的表观遗传.
DDM1充当关键调节器,将染色质重塑,基因素修饰和DNA甲基化联系起来,以保持表观遗传稳定.

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