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相关概念视频

Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
Coat Assembly and GTPases01:33

Coat Assembly and GTPases

Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
Receptor-Mediated Endocytosis01:20

Receptor-Mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...

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相关实验视频

Updated: Jun 19, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 27, 2011

克拉特林适配器真的非常适应.

Tom Kirchhausen1

  • 1Department of Cell Biology, Harvard Medical School and The Center for Blood Research, Boston, MA 02115, USA. kirchhausen@xta1200.harvard.edu

Cell
|June 28, 2002
PubMed
概括
此摘要是机器生成的。

克拉特林通路调解细胞内和生长因子下调. 研究人员确定了适配蛋白2 (AP-2) 内核的原子结构,揭示了它在将克拉特林层与货物蛋白连接中的作用.

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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
10:02

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

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相关实验视频

Last Updated: Jun 19, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 27, 2011

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

Published on: October 23, 2016

科学领域:

  • 细胞生物学 细胞生物学
  • 分子和结构生物学分子和结构生物学.

背景情况:

  • 克拉特林通路对于受体介导的内细胞分裂和调节生长因子信号传递至关重要.
  • 克拉斯林适配器将克拉斯林外层连接到跨膜载荷蛋白,以便将其分类为囊泡.

研究的目的:

  • 为了阐明适配蛋白2 (AP-2) 核心的原子结构.
  • 了解AP-2如何与细胞膜和载荷蛋白相互作用.

主要方法:

  • 用X射线晶体学或冷电子显微镜来确定原子结构.
  • 生物化学测试用于研究蛋白质与蛋白质相互作用.

主要成果:

  • 已经确定了AP-2核心的原子结构.
  • 该结构揭示了AP-2,膜和载荷蛋白细胞质尾巴之间的特定相互作用.

结论:

  • 解决的结构提供了关键的洞察力,对克拉林介导的内细胞分裂的分子机制.
  • 了解AP-2结构有助于研究受体贩运和细胞信号通路.