微阵列脱乙地图确定了酵母基因组脱乙酶的全基因组功能
Daniel Robyr1, Yuko Suka, Ioannis Xenarios
1Department of Biological Chemistry, UCLA School of Medicine and the Molecular Biology Institute, Boyer Hall, 90095, USA.
Cell
|June 28, 2002
概括
酵母中的基因组脱乙酶 (HDAC) 显示出分工,不同的酶如Rpd3,Hda1,Hos1/Hos3和Hos2调节特定的基因组和基因组区域,揭示了新的招募机制和功能.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 酵母遗传学 酵母遗传学
背景情况:
- 酵母具有五种相关的基因组脱乙酶 (HDACs),在整个基因组中具有很大程度上未知的功能.
- 了解HDAC的功能对于破译基因调节和表观遗传控制至关重要.
- 之前的研究仅在特定基因中确定了少数HDAC的功能.
研究的目的:
- 为了生成基因组范围的基因组脱乙酶 (HDAC) 酵素活性在酵母中的全基因组图.
- 确定不同酵母HDAC准的特定基因和基因组区域.
- 阐明酵母HDACs的不同作用和招募机制.
主要方法:
- 使用染色体免疫沉 (ChIP) 来分离与HDACs相关的DNA.
- 跨基因微阵列被用来生成全基因组的活动概况.
- 对HDAC目标和功能进行了比较分析.
主要成果:
- 通过新的招募途径,Rpd3和Hda1通过新的招募途径去乙基化大致不同的促进体和基因类.
- Hda1 deacetylates 亚端粒区域,影响参与葡萄糖生成和适应非葡萄糖碳源的基因.
- 相应地,Hos1/Hos3和Hos2优先准核糖体DNA和核糖体蛋白基因.
结论:
- 酵母基因脱乙酶 (HDACs) 在调节基因表达方面表现出显著的"分工".
- 乙化微阵列为绘制酶活性和发现功能专业化提供了强大的工具.
- 不同的HDAC在表观遗传调节中起着特殊的作用,包括异色素蛋白的形成和核糖体基因控制.
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