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In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
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VPAC(2) 受体对于小鼠上性核中的昼夜功能至关重要
Anthony J Harmar1, Hugh M Marston, Sanbing Shen
1Department of Neuroscience, University of Edinburgh, 1 George Square, United Kingdom. tony.harmar@ed.ac.uk
Cell
|June 28, 2002
概括
缺乏VPAC2受体的小鼠无法维持正常的昼夜节律或时钟基因表达. 这突显了神经信号传递在上核中对于昼夜功能的关键作用.
科学领域:
- 时间生物学 时间生物学
- 神经科学是一个神经科学.
- 分子生物学分子生物学
背景情况:
- 昼夜节律是由上神核 (SCN) 调节的.
- 脑垂体腺酸环酶激活多 (PACAP) 和血管活性肠 (VIP) 的神经参与SCN功能.
- VPAC2受体调解了PACAP和VIP的作用.
研究的目的:
- 研究VPAC2受体在昼夜节律调节中的作用.
- 确定VPAC2受体缺乏对SCN时钟基因表达和光诱导反应的影响.
主要方法:
- 在VPAC2受体基因中具有零突变的小鼠的生成和分析 (Vipr2(-/-)).
- 评估昼夜休息/活动行为.
- 在SCN中测量核心时钟基因 (mPer1,mPer2,mCry1) 和时钟控制基因 (氨酸血压素 - AVP) 的昼夜表达.
- 在夜间照明后评估mPer1和mPer2的急性诱导.
主要成果:
- Vipr2(-/-) 的小鼠表现出休息/活动的昼夜节律受损.
- 突变小鼠未能在SCN中显示关键时钟基因 (mPer1,mPer2,mCry1) 和AVP的正常昼夜表达模式.
- 在Vipr2(-/-) 小鼠中,mPer1和mPer2的夜间光感应表达被废除.
结论:
- VPAC2受体对于维持SCN中正常的昼夜节律和时钟基因表达至关重要.
- 由VPAC2受体介导的细胞间神经体信号传递,在SCN昼夜功能中起着关键作用.
- 这些发现强调了PACAP和VIP信号在调节生物时间方面的重要性.
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