埃纳/VASP蛋白质和actin线程封闭之间的对抗性调节纤维细胞运动
James E Bear1, Tatyana M Svitkina, Matthias Krause
1Massachusetts Institute of Technology, Department of Biology, Cambridge 02139, USA.
Cell
|June 28, 2002
概括
埃纳/VASP蛋白通过调节活性丝结构来影响细胞运动. 它们的缺失导致较慢但更持久的状凸,影响整体细胞运动.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 细胞移动性对生物过程至关重要,并依赖于由actin聚合驱动的乳突起.
- 众所周知,Ena/VASP蛋白在Listeria等病原体中促进了动因驱动的运动性,但矛盾的是,它抑制了细胞转位.
研究的目的:
- 调查Ena/VASP蛋白质在乳突起和细胞运动中的双重作用.
- 为了阐明Ena/VASP蛋白质如何在细胞运动过程中影响actin细胞骨架.
主要方法:
- 对Ena/VASP缺乏细胞和Ena/VASP过度表达细胞的分析.
- 显微镜检查状体动力学和actin网络架构.
- 在体外生化测试以确定Ena/VASP与actin纤维的相互作用.
主要成果:
- 缺乏Ena/VASP的乳体表现出更慢,更持久的突起和更短的,分支的纤维的改变了actin网络.
- 过多的Ena/VASP导致了lamellipodia内的更长,更少分支的活性丝.
- 实验室研究证实,Ena/VASP通过与刺刺的末端相互作用,促进了活性丝的延长.
结论:
- 埃纳/VASP蛋白质通过调节拉梅利波迪亚内的活性丝网的几何学来调节细胞运动,在调节细胞运动方面发挥着至关重要的作用.
- 这些发现解决了Ena/VASP对病原体和宿主细胞运动的对立作用的悖论.
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