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通过人工节点流来确定小鼠胚胎的左右图案
Shigenori Nonaka1, Hidetaka Shiratori, Yukio Saijoh
1Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada-oka, Suita, Osaka 565-0871, Japan. snonaka@fbs.osaka-u.ac.jp
Nature
|July 5, 2002
概括
这项研究表明,机械流体流动,而不仅仅是乳毛,对于在小鼠胚胎中打破左右体对称性至关重要. 应用人工流可以扭转正常的不对称性,证明了它在发育中的作用.
科学领域:
- 发展生物学 发展生物学
- 生物物理学的生物物理.
- 胚胎学 胚胎学
背景情况:
- 左右 (L-R) 非对称性是脊椎动物体平面发展的基础.
- 在胚胎中引发对称性破坏的确切机制在很大程度上是未知的.
- 节点流,在小鼠胚胎中由乳头驱动的向左流体运动,被假设是关键.
研究的目的:
- 调查机械流体流在建立L-R不对称性的直接作用.
- 为脊椎动物发育中的对称性破坏机制提供直接证据.
主要方法:
- 在受控的人工流体流动下培养小鼠胚胎的新系统的开发.
- 对野生类型和突变小鼠胚胎应用人造向右流.
- 对网站 (器官定位) 的观察,以应对操纵的节点流.
主要成果:
- 人工向右的流体流成功地逆转了野生类型胚胎的正常向左节点流.
- 这在被治疗的野生类型胚胎中诱导了逆位 (反向不对称).
- 人工流还纠正了小鼠胚胎的L-R模式,这些胚胎具有不动的乳毛,缺乏正常的节点流.
结论:
- 机械流体流动是脊椎动物左右身体不对称的关键决定因素.
- 这项研究提供了第一个直接的实验证据,证明了流体动力学在启动胚胎对称性破坏中的作用.
- 这些发现对理解与L-R模式相关的先天性异常有意义.
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