白领-1,一个DNA结合转录因子和一个光传感器
Qiyang He1, Ping Cheng, Yuhong Yang
1Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.
Science (New York, N.Y.)
|July 6, 2002
概括
白领-1 (WC-1) 蛋白质作为Neurospora crassa中的蓝光光受体,调节昼夜时钟的携带和其他光反应. 这一发现表明WC-1对于真菌的光感知至关重要.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 时间生物学 时间生物学
背景情况:
- 蓝光影响许多真菌生理过程.
- 在真菌中负责这些光反应的特定光受体仍然在很大程度上未被确定.
- 在Neurospora crassa中,已知Per-Arnt-Sim (PAS) 域转录因子白领-1 (WC-1) 调解所有光响应.
研究的目的:
- 为了研究WC-1蛋白在蓝光感知和生物钟调节中Neurospora crassa中的作用.
- 为了确定WC-1是否在这种真菌物种中起到蓝光光受体的作用.
主要方法:
- 对WC-1蛋白的基因操纵,特别是去除其光,氧或电压 (LOV) 域.
- 评估依赖光的生理反应,包括生理时钟的运行.
- 在突变菌株中研究昼夜时钟的温度带动.
- 对WC-1-WC-2蛋白质复合体进行生物化学分析,以确定相关染色体.
主要成果:
- 删除WC-1 LOV域取消了所有对光的反应,包括昼夜时钟被光所吸引.
- 在突变者中,frq基因的WC-1-介导的暗活化没有受到影响.
- 突变菌株的昼夜时钟仍然可能受到温度的影响.
- 经过净化WC-1-WC-2蛋白质复合体被发现含有氨酸-腺氨酸二核酸 (FAD) 在石化量.
结论:
- WC-1 作为Neurospora crassa.中的主要蓝光光受体.
- 这种WC-1的光受体活性对于昼夜时钟的光携带和其他光介导反应至关重要.
- 在WC-1复合体中FAD的存在支持其作为黄蛋白光受体的作用.
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