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相关概念视频

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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相关实验视频

Updated: Jul 7, 2026

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::&beta;-glucuronidase Reporter Gene Expression
10:24

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression

Published on: June 11, 2010

通过从A. lyrata转移两个S位基因来生成自我不兼容的Arabidopsis thaliana.

Mikhail E Nasrallah1, Pei Liu, June B Nasrallah

  • 1Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA. men4@cornell.edu

Science (New York, N.Y.)
|July 13, 2002
PubMed
概括

从Arabidopsis lyrata转移到Arabidopsis thaliana的耻辱受体激酶SRK和花粉配体SCR的基因转移恢复了自我不相容性. 这项研究确定了控制植物交配系统的关键基因.

科学领域:

  • 植物生殖生物学 植物生殖生物学
  • 遗传学和基因组学 遗传学和基因组学
  • 进化植物学的植物学.

背景情况:

  • 植物交配系统经常在交叉受精和自我受精之间进行过渡.
  • 这些交配系统过渡的遗传基础在很大程度上是未知的.
  • 自我不相容性 (SI) 是许多植物物种防止自我受精的关键机制.

研究的目的:

  • 研究植物自我不相容性 (SI) 的遗传基础.
  • 为了确定来自自我不兼容的物种的特定基因是否可以将SI赋予自我生育的物种.
  • 探索交配系统过渡的进化潜力.

主要方法:

  • 来自Arabidopsis lyrata的耻辱受体激酶SRK及其由花粉传播的配体SCR的基因转移.
  • 引入这些基因到自我肥沃的物种Arabidopsis thaliana中,该物种缺乏功能性正义基因.
  • 在转变的Arabidopsis thaliana中对自我不相容的表型分析.

主要成果:

  • 从A. lyrata成功转移SRK和SCR的基因,使得A. thaliana获得了自我不相容的表型.
  • 这表明,用于排斥花粉的核心信号通路被保留了.
  • 阿拉比多普西斯·塔利亚纳 (Arabidopsis thaliana) 是一种自我肥沃的物种,可以被改造为SI分析.

更多相关视频

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napus
08:52

Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napus

Published on: December 22, 2023

相关实验视频

Last Updated: Jul 7, 2026

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::&beta;-glucuronidase Reporter Gene Expression
10:24

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression

Published on: June 11, 2010

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napus
08:52

Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napus

Published on: December 22, 2023

结论:

  • 污名受体激酶SRK和花粉配体SCR足以建立自我不相容性.
  • 对于花粉抑制的保存信号级联突出显示了交配系统进化的遗传基础.
  • 阿拉比多普西斯塔利亚纳为进一步研究自我不兼容机制提供了一个可操作的系统.