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相关概念视频

Paracrine Signaling01:21

Paracrine Signaling

Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Necrosis01:16

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
Pigmentation01:19

Pigmentation

The color of the skin is influenced by a number of pigments, including melanin, carotene, and hemoglobin. Recall that melanin is produced by cells called melanocytes, which are found scattered throughout the stratum basale of the epidermis. The melanin is transferred to the keratinocytes via melanosomes.
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...

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Updated: Jul 5, 2026

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
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Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS

Published on: December 21, 2011

尼菲迪平通过血管光滑肌肉细胞依赖的途径间接上调内皮细胞中超氧化物脱酶的表达.

Keisuke Fukuo1, Jin Yang, Osamu Yasuda

  • 1Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan. fukuo@geriat.med.osaka-u.ac.jp

Circulation
|July 18, 2002
PubMed
概括

像尼菲迪平这样的抗体通过增加内皮细胞 (ECs) 中的超氧化失调酶 (SOD) 来改善内皮功能. 这种效应是由血管光滑肌细胞 (VSMCs) 介导的,它们释放血管内皮生长因子 (VEGF).

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科学领域:

  • 心血管研究研究心血管研究
  • 内皮细胞生物学 内皮细胞生物学
  • 顺滑肌细胞功能 顺滑肌细胞功能

背景情况:

  • 已知抗体可以改善心血管疾病中的内皮功能障碍.
  • 内皮细胞中抗体的特定受体仍未确定.
  • 血管光滑肌细胞 (VSMCs) 假设调解抗体对内皮细胞 (ECs) 的作用.

研究的目的:

  • 调查VSMC是否调节内皮超氧化物失调酶 (SOD) 活动和表达的对手尼菲迪平的调节.
  • 阐明血管内皮生长因子 (VEGF) 在这个过程中的作用.

主要方法:

  • 关于EC和VSMC的共同文化研究.
  • 用尼菲迪平 (nifedipine) 治疗,尼菲迪平是一种抗体.
  • 测量SOD活动和表达.
  • 对血管内皮生长因子 (VEGF) 生产的评估.
  • 布拉迪基宁B2受体对抗剂 (HOE-140) 和VEGF中和抗体的使用.

主要成果:

  • 当与VSMC共同培养时,nifedipine在EC中调节了SOD表达和活性,但当单独培养时不会.
  • 尼菲迪平刺激了VSMCs的VEGF产量,而HOE-140则阻止了这种产量.
  • 在EC中,VEGF中和抗体抑制了尼菲迪平诱导的SOD上调.
  • 再组合VEGF在EC中增加了SOD表达.
  • VSMC超增强了EC中的氧化 (NO) 生产,这种影响取决于SOD.

结论:

  • 抗体尼菲迪平间接上调内皮SOD的表达.
  • 这种上调是通过邻近的VSMCs的VEGF刺激来调节的.
  • 这些发现提供了关于抗体在心血管疾病中的有益机制的见解.