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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

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Published on: August 27, 2014

用DNA和蛋白质写作,使用纳米管片进行受控传递.

Andreas Bruckbauer1, Liming Ying, Alison M Rothery

  • 1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.

Journal of the American Chemical Society
|July 26, 2002
PubMed
概括

我们开发了一种新的纳米管子方法,用于精确地将生物分子沉积在表面上. 这种技术可以控制DNA和蛋白质的输送,以创建定义的特征.

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科学领域:

  • 生物技术是生物技术.
  • 表面科学是一门学科.
  • 纳米技术 纳米技术

背景情况:

  • 对生物分子的控制沉积对于各种应用至关重要,包括生物传感器和微阵列.
  • 现有的方法在控制交付率和特征大小方面往往缺乏精度.

研究的目的:

  • 提出一种新的,用于控制生物分子在表面沉积的一般方法.
  • 为了证明对递送速率和沉积分子的位置的精细控制.

主要方法:

  • 利用一个在离子溶液中运行的纳米管,并应用了控制分子流量的潜力.
  • 采用离子电流反来调节纳米管距离表面的距离.
  • 在玻璃幻灯片上沉积光标记的DNA和蛋白质G.

主要成果:

  • 在定义的位置实现生物分子的受控沉积.
  • 通过将生物化DNA沉积在斯特雷普塔维丁表面上,创建了830nm的特征.
  • 通过将蛋白质G沉积在带正电荷的玻璃表面上,产生了1.3微米的斑点.

结论:

  • 纳米管子方法提供了一种多功能和精确的方法,用于用生物分子进行表面图案.
  • 这种技术允许创建特定尺寸的特征,具有高空间精度.
  • 该方法在制造纳米生物设备和平台方面具有潜在的应用.