蛋白质拼接是由一个小分子触发的
1Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Journal of the American Chemical Society
|August 1, 2002
概括
研究人员开发了一种使用小分子控制蛋白质功能的新方法. 这种技术利用蛋白质拼接,使得蛋白质活性在响应拉巴胺时能够精确的时间调节.
科学领域:
- 化学生物学 化学生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 通过小分子控制蛋白质功能,可以提供难以通过遗传方法实现的时间精度.
- 目前的策略通常涉及直接与蛋白质活性位点结合或诱导结构变化.
- 通过小分子改变蛋白质的主要结构提供了一个替代的控制机制.
研究的目的:
- 引入一种使用小分子控制蛋白质功能的新技术.
- 为了利用蛋白质拼接来诱导蛋白质活动的控制.
- 开发一种小分子介导蛋白调节的可通用方法.
主要方法:
- 利用蛋白质拼接,这是一个自然的翻译后过程.
- 设计一个由小分子拉巴胺触发蛋白质拼接的系统.
- 开发一种独立于特定N-和C-extein序列的方法.
主要成果:
- 展示了一种技术,使得蛋白质拼接只能在拉巴胺的存在下进行.
- 建立了用于小分子诱导蛋白质功能控制的新机制.
- 展示了在不同的蛋白质标中广泛应用的潜力.
结论:
- 开发的技术为用小分子控制蛋白质功能提供了一个可通用的平台.
- 这种方法提供了精确的蛋白质活性时间调节.
- 这种方法通过引入一种用于蛋白质操纵的新工具来推进化学遗传学领域.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...


