mTOR与猛禽相互作用,形成一种对营养素敏感的复合体,向细胞生长机械发出信号
Do-Hyung Kim1, D D Sarbassov, Siraj M Ali
1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
Cell
|August 2, 2002
概括
拉巴胺素 (mTOR) 途径的机械性标调节细胞生长. 这项研究确定猛禽是一种关键蛋白质,与mTOR形成复合体,根据营养的可用性控制细胞大小.
科学领域:
- 细胞生物学 细胞生物学
- 分子信号传输的方法
- 生物化学 生物化学
背景情况:
- 拉巴胺素 (mTOR) 途径的机械性标对于调节细胞生长以应对营养和激素至关重要.
- mTOR是免疫抑制药物拉巴胺的目标.
- 通过mTOR感知营养水平的精确机制仍然不完全理解.
研究的目的:
- 为了研究保护蛋白质猛禽在mTOR信号通路中的作用.
- 阐明猛禽如何与mTOR相互作用并影响其活动.
- 确定mTOR-猛禽复合体对营养感应和细胞大小调节的贡献.
主要方法:
- 在mTOR和猛禽之间形成蛋白质复合物的生物化学分析.
- 在各种营养条件下测量mTOR激酶活性.
- 研究猛禽对S6K1和细胞大小等下游效应者的影响.
主要成果:
- mTOR与猛禽形成了一个固体测量复合体,猛禽是mTOR通路中保存的蛋白质.
- 猛龙对S6K1,细胞大小维护和mTOR蛋白表达的营养刺激信号进行积极调节.
- 猛禽与mTOR的关联负面调节了mTOR激酶活性.
- 营养缺乏和线粒体脱稳定了mTOR-猛禽复合体,并抑制了mTOR激酶活性.
结论:
- 猛禽是mTOR通路的关键组成部分,作为营养可用性的传感器.
- mTOR - 猛禽复合体在调节细胞大小以应对营养水平方面发挥着关键作用.
- 了解mTOR-猛禽相互作用,可以了解细胞生长控制和潜在的治疗点.
相关概念视频
03:03mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
01:19Interactions Between Signaling Pathways
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Convergence and divergence, and cross-talk between signaling pathways
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Convergence and divergence, and cross-talk between signaling pathways
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01:26Receptor Tyrosine Kinases
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01:07MAPK Signaling Cascades
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01:22PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...
03:03mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...