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相关概念视频

What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Structure of a Gene01:30

Structure of a Gene

A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...

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相关实验视频

Updated: Jun 25, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

修改基因表达程序通过改变核心促销器染色质结构来修改基因表达程序.

Stavros Lomvardas1, Dimitris Thanos

  • 1Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA.

Cell
|August 2, 2002
PubMed
概括

病毒感染通过增强酶组合和染色体重塑触发IFN-β基因激活. 预定位一个关键的核细胞改变基因表达时间和特异性,突出增强细胞和染色质结构之间的相互作用.

科学领域:

  • 分子生物学分子生物学
  • 免疫学 免疫学 免疫学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 干扰素-β (IFN-β) 基因的转录激活对于抗病毒反应至关重要.
  • 这一过程涉及到增强酶体复杂和动态染色体重塑在基因的促进器的形成.
  • 一个关键的步骤是重新定位通常阻断核心促进体的核酶.

研究的目的:

  • 调查核细胞定位在调节IFN-β基因表达程序中的作用.
  • 为了确定核酶体的预定位是否会影响对病毒感染的时间和信号特异性转录反应.

主要方法:

  • 试验性操纵核细胞位在IFN-β促进体的位置.
  • 基因表达模式的分析病毒挑战后在细胞中改变了染色质结构.
  • 对转录激活动态和特异性的评估.

主要成果:

  • 将IFN-β促进子阻断核酶的先发性滑向下游位置显著改变了该基因的表达特征.
  • 与标准诱导途径相比,转录反应的时间和信号特异性发生了明显的变化.
  • 这表明,最初的染色质结构影响了随后的基因表达程序.

结论:

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Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
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Published on: March 10, 2023

相关实验视频

Last Updated: Jun 25, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
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Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

  • 特定增强体复合体和局部染色体结构之间的动态相互作用决定了精确的基因表达程序.
  • 特定的局部染色体结构对于建立和维护基因表达程序的身份至关重要.
  • 这一发现提供了关于在表观遗传层面上控制先天免疫反应的调节机制的见解.