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相关概念视频

Seed Structure and Early Development of the Sporophyte02:33

Seed Structure and Early Development of the Sporophyte

Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

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相关实验视频

Updated: Jul 6, 2026

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
09:23

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Published on: January 28, 2011

DEMETER是一种DNA糖基酶域蛋白质,是内精基因印记和种子活性的必要条件.

Yeonhee Choi1, Mary Gehring, Lianna Johnson

  • 1Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

Cell
|August 2, 2002
PubMed
概括

对于种子发育至关重要的DEMETER (DME) 基因激活了母亲的基因表达. 这种DNA糖酶通过调节印制基因来控制胚胎和内的形成.

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Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
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Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
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相关实验视频

Last Updated: Jul 6, 2026

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
09:23

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Published on: January 28, 2011

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
08:05

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Published on: June 7, 2013

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
06:28

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

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科学领域:

  • 植物分子生物学 植物分子生物学
  • 发育生物学是发展生物学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 阿拉比多普西斯 (Arabidopsis thaliana) 是研究植物遗传学的模型生物.
  • 种子发育是一个复杂的过程,涉及母亲和父亲的贡献.
  • 印制的基因,仅从一个亲代基因表达出来,在种子发育中发挥关键作用.

研究的目的:

  • 调查DEMETER (DME) 基因在控制Arabidopsis的胚胎和内的发育中的作用.
  • 了解DME调节印记基因表达的机制.
  • 阐明DNA糖系酶在植物发育中的作用.

主要方法:

  • 隔离了影响种子发育的突变,在Arabidopsis.
  • 使用分子技术分析基因表达模式.
  • 通过子宫外表达研究来研究DME的功能.

主要成果:

  • 种子的生存能力取决于DEMETER (DME) 基因的母体副本.
  • DME编码了DNA糖酶,并在雌性性体细胞的中央细胞中表达.
  • DME对于在中细胞和内精子中印制的MEDEA (MEA) 基因的母体表达至关重要.
  • 宫外DME表达激活了被沉默的父性MEA等位基因,并杀了MEA促进体.

结论:

  • 基因糖酶DME激活了母亲在细胞中印制的基因的表达.
  • 在种子发育过程中,DME在调节基因印记方面发挥着至关重要的作用.
  • 了解DME的功能,可以了解植物胚胎发生的表观遗传控制.