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Updated: Jan 19, 2026

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在试验室内选择具有核糖体显示的催化活性与核糖体显示

Patrick Amstutz1, Joelle N Pelletier, Armin Guggisberg

  • 1Biochemisches Institut, Universität Zürich, Winterthurerstrasse 190, Switzerland.

Journal of the American Chemical Society
|August 9, 2002
PubMed
概括

这项研究引入了第一个使用核糖体显示的酶催化活性在体外选择. 这种方法可以直接选择酶功能,扩大酶发现的潜在序列空间.

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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