培养无处不在的SAR11海洋细菌浮游生物分类
Michael S Rappé1, Stephanie A Connon, Kevin L Vergin
1Department of Microbiology, Oregon State University, Corvallis, Oregon 97331, USA.
Nature
|August 9, 2002
概括
科学家们成功培养了SAR11细菌,这是一种无处不在的海洋微生物,以前没有培养过. 这一突破允许进一步研究这些丰富,但难以捉摸的海洋微生物.
科学领域:
- 微生物学 微生物学
- 海洋生物学 海洋生物学
- 基因组学就是基因组学.
背景情况:
- 一个阿尔法-蛋白质细菌系的SAR11类,是海洋细菌浮游生物群落的主要组成部分.
- 尽管SAR11无处不在,但仍无法使用传统方法进行培养,这阻碍了对其生态作用的研究.
- 培养独立的调查显示,SAR11包含海水中已识别的rRNA基因的很大一部分 (26%).
研究的目的:
- 为了隔离和培养SAR11类的代表.
- 为了克服长期以来培养这种丰富的海洋微生物的挑战.
- 为了使未来对SAR11的生理学和地球化学进行研究.
主要方法:
- 细胞培养的高通量隔离.
- 自然微生物群落的稀释到低营养的介质中.
- 成功培养,通过和冷保存分离物.
主要成果:
- 获得了18种SAR11代表的培养.
- 11种文化成功通过并冷保存.
- 孤立的SAR11细胞是最小的自由活细胞之一,体积约为0.01μm3.
结论:
- 成功种植SAR11为研究这个生态重要海洋群体开辟了新的途径.
- 这一成就为研究人员提供了可行的培养物,用于详细的生理和代谢研究.
- 这些微小细胞的隔离有助于我们理解微生物生活在寡头性海洋环境.
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