Wnt-11激活非正规的Wnt信号通路是心脏生成所需的
Petra Pandur1, Matthias Läsche, Leonard M Eisenberg
1[1] Abt Entwicklungsbiochemie, Junior group SFB 271, Universität Göttingen, Humboldtallee 23, D-37073 Göttingen, Germany [2] Abt Biochemie, Universität Ulm, Albert-Einstein-Allee-11, D-89081 Ulm, Germany.
Nature
|August 9, 2002
概括
克塞诺普斯Wnt-11对于脊椎动物的心脏形成至关重要. 该基因激活非正规的Wnt信号,这对心脏发育至关重要,并在物种中得到保护.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 脊椎动物的心脏形成涉及复杂的,时间调节的信号通路.
- 在Xenopus laevis中,胃化过程中的心脏特异性取决于背唇和内皮的信号.
- Xenopus Wnt-11表现出与心脏特征相关的表达模式,这表明它在心脏发育中的作用.
研究的目的:
- 为了研究XWnt-11在Xenopus心脏形成中的作用.
- 为了确定Wnt-11在心脏发育中的功能是否在较高的脊椎动物中得到保护.
- 为了阐明XWnt-11在心脏发生过程中介导的信号通路.
主要方法:
- 在Xenopus胚胎中的功能损失和功能增益实验.
- 在胚胎扩展体中诱导收缩性表型.
- 用小鼠Wnt-11条件介质治疗小鼠P19干细胞.
主要成果:
- 在Xenopus胚胎中,XWnt-11对于心脏形成是不可或缺的.
- XWnt-11可以诱导胚胎扩展体中的收缩性心脏表型.
- 鼠Wnt-11在P19细胞中触发心脏发生,表明功能得到保护.
- XWnt-11使用非正规的Wnt信号,独立于β-catenin,涉及PKC和JNK.
结论:
- XWnt-11是Xenopus.11中心脏发育的关键调节者.
- 非正规的Wnt信号对于心脏发育计划至关重要.
- 在心脏发生过程中Wnt-11的作用是进化保守的.
相关概念视频
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...