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CNS Stimulants: Cocaine, Amphetamines and Cannabinoids01:24

CNS Stimulants: Cocaine, Amphetamines and Cannabinoids

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CNS stimulants, such as cocaine, amphetamines, and cannabinoids, have varying structures and mechanisms of action that lead to different therapeutic effects and side effects. Cocaine, with its molecular formula C17H21NO4, is a tropane alkaloid and a tertiary amino compound. It has two chemical forms: the hydrochloride salt and the "freebase." The former is in powder form, while the latter involves removing the hydrochloride salt to create a form that can be smoked. Cocaine exerts its...
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Lewis Acids and Bases02:33

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In 1923, G. N. Lewis proposed a generalized definition of acid-base behavior in which acids and bases are identified by their ability to accept or to donate a pair of electrons and form a coordinate covalent bond.
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...
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Weak Base Solutions03:21

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Some compounds produce hydroxide ions when dissolved by chemically reacting with water molecules. In all cases, these compounds react only partially and so are classified as weak bases. These types of compounds are also abundant in nature and important commodities in various technologies. For example, global production of the weak base ammonia is typically well over 100 metric tons annually, being widely used as an agricultural fertilizer, a raw material for chemical synthesis of other...
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One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
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Salts with Acidic Ions
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
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基于Aptamer的色度测量探针用于可卡因.

Milan N Stojanovic1, Donald W Landry

  • 1Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, Box 84, 630 West 168th Street, New York, New York 10032, USA. mns18@columbia.edu

Journal of the American Chemical Society
|August 15, 2002
PubMed
概括
此摘要是机器生成的。

一个使用抗可卡因吸收剂和染料的新色度传感器检测可卡因. 传感器通过染料位移来工作,允许视觉检测溶液中的可卡因.

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科学领域:

  • 生物化学 生物化学
  • 分析化学 分析化学
  • 材料科学 材料科学 材料科学

背景情况:

  • 开发敏感和选择性的可卡因检测方法对于临床和法医应用至关重要.
  • 亚胺为生物传感器开发提供独特的分子识别能力.
  • 色度传感器提供了一个简单而经济的检测平台.

研究的目的:

  • 开发和描述一种用于可卡因检测的新型色度传感器.
  • 为了研究阿普塔米尔染料复合物的感知机制.
  • 为了评估传感器在相关度范围内的性能.

主要方法:

  • 在抗可卡因阿巴和二甲基二甲基三碳酸染料之间形成复合物.
  • 谱光测量分析以确定可卡因结合后在760nm的吸收率的变化.
  • 视觉观测染料沉和超浮物脱色的定性检测.

主要成果:

  • 胺染料复合体的功能是可卡因的色度传感器.
  • 在760nm的吸收率下降与可卡因度 (2-600μM) 相对应.
  • 感知机制涉及可卡因对染料的分子间移位,导致染料沉和视觉颜色变化.

结论:

  • 开发出来的阿普塔默染料复合体作为可卡因的有效色度传感器.
  • 传感器提供了一个简单的,视觉检测方法,由于染料的不溶性.
  • 这种方法为快速检测可卡因提供了一个有希望的平台.