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Updated: Jul 18, 2026

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用自结合灭探头对细菌中的RNA进行成像

Shinsuke Sando1, Eric T Kool

  • 1Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.

Journal of the American Chemical Society
|August 15, 2002
PubMed
概括

新的定量调整 (QUAL) 探针可以直接检测细菌RNA. 这些探针在结合大肠杆菌中的特定16SrRNA序列时发光,消除了对酶或洗步骤的需求.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...