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相关概念视频

Mismatch Repair01:36

Mismatch Repair

Overview
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
Genome Copying Errors02:46

Genome Copying Errors

DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

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相关实验视频

Updated: Jul 10, 2026

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
11:52

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level

Published on: April 23, 2016

人类l1逆转换与体内遗传不稳定性有关.

David E Symer1, Carla Connelly, Suzanne T Szak

  • 1Department of Molecular Biology and Genetics, John Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Cell
|August 15, 2002
PubMed
概括

人类L1逆转移子会导致遗传不稳定,导致逆转,删除和插入. 新的研究揭示了L1插入结构的机制模型,有助于基因组稳定性研究.

科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 逆转移体,就像人类的L1元素一样,是移动的遗传元素,对真核生物基因组进化产生了重大影响.
  • 了解L1逆转换对体细胞的影响对于理解基因组可塑性和疾病发展至关重要.

研究的目的:

  • 开发一种基因系统,有效地在体细胞中恢复新的L1插入.
  • 描述L1逆转换的结构后果,并确定相关的遗传不稳定性.
  • 提出一种解释观察到的L1插入结构的机械模型.

主要方法:

  • 开发一种新的遗传系统,以捕捉体细胞中的新L1插入.
  • 使用分子和基因组技术分析了42个回收的L1整合物.
  • 对L1插入结构和侧翼序列的比较分析.

主要成果:

  • 成功恢复了42个新的L1整合物,模仿灵长类动物的L1积累.
  • 在L1逆转换和各种遗传不稳定性之间显示出强烈的关联,包括倒置,插入,删除和5'转导.
  • 确定了L1供体和点之间共享的短相同序列,提供了机械洞察力.

结论:

更多相关视频

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
11:04

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR
10:54

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Published on: July 27, 2019

相关实验视频

Last Updated: Jul 10, 2026

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
11:52

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level

Published on: April 23, 2016

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
11:04

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR
10:54

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Published on: July 27, 2019

  • 人类L1逆转换是体质遗传不稳定的重要驱动因素.
  • 鉴定出的基因变异为L1在塑造基因组架构中的作用提供了实验证据.
  • 一个涉及捐赠体和点共享序列的拟议机械模型解释了L1插入模式.