一个RNA结合蛋白的调节由酸激活蛋白激酶
Jiaxu Li1, Toshinori Kinoshita, Sona Pandey
1Biology Department, The Pennsylvania State University, 208 Mueller Laboratory, University Park, Pennsylvania 16802, USA. jiax_li@hms.harvard.edu
Nature
|August 16, 2002
概括
植物激素酸调节压力反应. 酸激活蛋白激酶 (AAPK) 化AKIP1,控制其RNA结合应激保护蛋白.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 蛋白激酶在植物和动物的压力信号通路中起着至关重要的作用.
- 酸是一种关键的植物激素,它调解了对干旱和度等环境压力的反应.
- 护卫细胞中的酸激活蛋白激酶 (AAPK) 调节离子通道.
研究的目的:
- 研究AAPK相互作用蛋白1 (AKIP1) 在酸信号传递中的作用.
- 确定AKIP1是否是AAPK的基质,以及这种相互作用如何影响基因表达.
- 探索AKIP1对酸的反应中的亚核定位和动态.
主要方法:
- 生物化学试验证实AKIP1是AAPK基质.
- 分析AKIP1酸化及其对信使RNA结合的影响.
- 显微镜技术可视化AKIP1在护卫细胞核中的定位.
主要成果:
- AKIP1被确定为AAPK的基质.
- 通过AAPK对AKIP1的酸化对于其与脱水素信使RNA的结合至关重要.
- 酸处理会诱导护卫细胞中形成含有AKIP1的亚核点.
结论:
- 异质核RNA结合蛋白对酸化依赖的RNA标选择是一种保存的真核细胞机制.
- 植物激素可以调节应激反应期间的蛋白质动态和亚核组织.
关键词:
非编程性的非编程性.相关概念视频
Nucleic acids
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Nucleic Acids
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Editing
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA Structure
DNA has a double-helix structure. The...


