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相关概念视频

Complementary DNA01:44

Complementary DNA

Overview
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

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相关实验视频

Updated: Jul 11, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

通过DNA模板编程的多步小分子合成.

Zev J Gartner1, Matthew W Kanan, David R Liu

  • 1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

Journal of the American Chemical Society
|August 29, 2002
PubMed
概括

研究人员开发了新的DNA模板合成方法来制造小分子. 这一突破使多步合成成为可能,将DNA序列转化为功能产品,用于潜在的前生物应用.

科学领域:

  • 合成生物学 合成生物学
  • 有机化学 有机化学
  • 分子进化的分子进化.

背景情况:

  • DNA模板合成对于演化合成分子至关重要.
  • 以前的方法缺乏有效的多步合成和产品隔离策略.

研究的目的:

  • 开发用于特定序列DNA模板合成的通用链接器和净化策略.
  • 为了实现第一个多步核酸模板小分子合成.

主要方法:

  • 开发了新的链接器和净化技术.
  • 利用DNA模板进行序列特定合成.
  • 执行了翻译,选择和放大的代循环.

主要成果:

  • 成功分离和净化DNA模板反应产物.
  • 首次实现了由核酸模拟的两个不同的小分子的多步合成.
  • 证明了分离产品能够经历随后的DNA模板反应的能力.

结论:

  • 建立了一个可靠的方法来将DNA序列转化为多步合成产品.
  • 提供了实验证据,支持涉及核酸模板反应的前生物合成模型.

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Design and Synthesis of a Reconfigurable DNA Accordion Rack
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Design and Synthesis of a Reconfigurable DNA Accordion Rack

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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

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相关实验视频

Last Updated: Jul 11, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

  • 开辟了创造复杂分子和理解早期生命化学的新途径.