诺胺生物合成:可能是氨基基酸盐路径中原子的来源
1Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
Journal of the American Chemical Society
|September 5, 2002
概括
加诺胺生物合成被确定为aminoshikimate通路中的原子的来源. 这项研究证明了可诺胺6酸盐.
科学领域:
- 生物化学 生物化学
- 代谢途径 代谢途径
- 微生物生物合成
背景情况:
- 在生物化学研究中,原子在aminoshikimate通路中的起源一直是一个未解决的问题.
- 之前的研究表明,3-氨基-3-脱氧-D-果糖-6-酸盐作为*Amycolatopsis mediterranei*的前体.
研究的目的:
- 为了调查天然产品kanosamine是否作为3-amino-3-deoxy-D-fructose 6-phosphate的前体.
- 为了阐明纳入氨基基酸盐路径的原子的生物合成来源.
主要方法:
- 康诺山胺6-酸盐的化学酶合成.
- 合成的可诺山胺6酸盐与*A. mediterranei*无细胞溶酸盐,D- рибо5酸盐和烯酸酸的化.
- 用*A. mediterranei*无细胞溶酸盐,谷氨酸和NAD化UDP葡萄糖以观察索胺的形成.
主要成果:
- 在与kanosamine 6-phosphate进行化后,观察到4-amino-3,4-dideoxy-D-arabino-heptulosonic acid 7-phosphate和3-amino-5-hydroxybenzoic acid的形成.
- 诺胺是由UDP-葡萄糖在无细胞溶解酸中形成的.
- 证实了可诺山胺6-酸盐的生物转化为关键的氨基基酸盐路径中间体.
结论:
- 生物转化UDP-葡萄糖到kanosamine和随后的转化kanosamine-6-酸盐到途径中间体强烈表明kanosamine生物合成作为源.
- 这项研究解决了长期以来关于原子在aminoshikimate通路中的起源的问题.
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