缺陷的费洛蒙反应在小鼠缺少一个集群的vomeronasal受体基因
Karina Del Punta1, Trese Leinders-Zufall, Ivan Rodriguez
1The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Nature
|September 6, 2002
概括
鼻器官 (VNO) 检测到激素,但其受体是未知的. 在小鼠中删除V1r基因损害了激素检测和改变了社会行为,证实了V1rr.
科学领域:
- 神经科学是一个神经科学.
- 遗传学 遗传学 是一个
- 动物行为 动物行为
背景情况:
- 哺乳动物的口腔鼻腔器官 (VNO) 检测到激素,这对社会和生殖行为至关重要.
- 负责在VNO中检测费洛蒙的分子受体仍然在很大程度上未被定义.
- 候选费洛蒙受体由V1r和V2r基因超级家族编码,在VNO感觉神经元中表达.
研究的目的:
- 研究V1r基因在激素检测和VNO依赖行为中的功能作用.
- 为V1r受体作为费洛蒙受体提供功能性证据.
主要方法:
- 利用染色体工程来删除600基基基因组区域,其中包含小鼠中的16个完整的V1r基因.
- 评估突变小鼠的行为变化,重点关注雄性性行为和母性侵略.
- 对VNO表皮进行了电生理学记录,以评估对费洛蒙配体的反应.
主要成果:
- 突变小鼠在特定的VNO-依赖性行为中表现出显著的缺陷,包括改变的男性性行为和母性侵略.
- 电生理学分析显示,突变小鼠的VNO表皮对特定的费洛蒙配体没有可检测的反应.
- 删除影响了大约12%的V1r基因库,包括两个V1r基因家族.
结论:
- 在V1r缺乏的小鼠中观察到的行为障碍和化学感知缺陷强烈支持V1r受体在激素检测中的作用.
- 这项研究提供了第一个功能性证据,将V1r基因与费罗蒙反应和VNO介导的行为联系起来.
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